Serveur d'exploration SRAS

Attention, ce site est en cours de développement !
Attention, site généré par des moyens informatiques à partir de corpus bruts.
Les informations ne sont donc pas validées.

Disease-specific B cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens

Identifieur interne : 005576 ( Main/Exploration ); précédent : 005575; suivant : 005577

Disease-specific B cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens

Auteurs : I-Ju Liu [Taïwan] ; Po-Ren Hsueh [Taïwan] ; Chin-Tarng Lin [Taïwan] ; Chien-Yu Chiu [Taïwan] ; Chuan-Liang Kao [Taïwan] ; Mei-Ying Liao [Taïwan] ; Han-Chung Wu [Taïwan]

Source :

RBID : ISTEX:C2A0D758774998C51462273113E7DFDB9C630BC7

Descripteurs français

English descriptors

Abstract

Severe acute respiratory syndrome (SARS) has emerged as a highly contagious, sometimes fatal disease. To find disease-specific B cell epitopes, phage-displayed random peptide libraries were panned on serum immunoglobulin (Ig) G antibodies from patients with SARS. Forty-nine immunopositive phage clones that bound specifically to serum from patients with SARS were selected. These phageborne peptides had 4 consensus motifs, of which 2 corresponded to amino acid sequences reported for SARS-associated coronavirus (SARSCoV). Synthetic peptide binding and competitive-inhibition assays further confirmed that patients with SARS generated antibodies against SARS-CoV. Immunopositive phage clones and epitope-based peptide antigens demonstrated clinical diagnostic potential by reacting with serum from patients with SARS. Antibody-response kinetics were evaluated in 4 patients with SARS, and production of IgM, IgG, and IgA were documented as part of the immune response. In conclusion, B cell epitopes of SARS corresponded to novel coronavirus. Our epitope-based serologic test may be useful in laboratory detection of the virus and in further study of the pathogenesis of SARS.

Url:
DOI: 10.1086/422753


Affiliations:


Links toward previous steps (curation, corpus...)


Le document en format XML

<record>
<TEI wicri:istexFullTextTei="biblStruct">
<teiHeader>
<fileDesc>
<titleStmt>
<title>Disease-specific B cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens</title>
<author>
<name sortKey="Liu, I Ju" sort="Liu, I Ju" uniqKey="Liu I" first="I-Ju" last="Liu">I-Ju Liu</name>
</author>
<author>
<name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name>
</author>
<author>
<name sortKey="Lin, Chin Tarng" sort="Lin, Chin Tarng" uniqKey="Lin C" first="Chin-Tarng" last="Lin">Chin-Tarng Lin</name>
</author>
<author>
<name sortKey="Chiu, Chien Yu" sort="Chiu, Chien Yu" uniqKey="Chiu C" first="Chien-Yu" last="Chiu">Chien-Yu Chiu</name>
</author>
<author>
<name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name>
</author>
<author>
<name sortKey="Liao, Mei Ying" sort="Liao, Mei Ying" uniqKey="Liao M" first="Mei-Ying" last="Liao">Mei-Ying Liao</name>
</author>
<author>
<name sortKey="Wu, Han Chung" sort="Wu, Han Chung" uniqKey="Wu H" first="Han-Chung" last="Wu">Han-Chung Wu</name>
</author>
</titleStmt>
<publicationStmt>
<idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:C2A0D758774998C51462273113E7DFDB9C630BC7</idno>
<date when="2004" year="2004">2004</date>
<idno type="doi">10.1086/422753</idno>
<idno type="url">https://api.istex.fr/ark:/67375/HXZ-P5B21G08-N/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000183</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000183</idno>
<idno type="wicri:Area/Istex/Curation">000183</idno>
<idno type="wicri:Area/Istex/Checkpoint">001C54</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001C54</idno>
<idno type="wicri:doubleKey">0022-1899:2004:Liu I:disease:specific:b</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7109815</idno>
<idno type="RBID">PMC:7109815</idno>
<idno type="wicri:Area/Pmc/Corpus">001165</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001165</idno>
<idno type="wicri:Area/Pmc/Curation">001165</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001165</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001682</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001682</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:15272409</idno>
<idno type="wicri:Area/PubMed/Corpus">002C47</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002C47</idno>
<idno type="wicri:Area/PubMed/Curation">002C47</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002C47</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002D36</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D36</idno>
<idno type="wicri:Area/Ncbi/Merge">000967</idno>
<idno type="wicri:Area/Ncbi/Curation">000967</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000967</idno>
<idno type="wicri:doubleKey">0022-1899:2004:Liu I:disease:specific:b</idno>
<idno type="wicri:Area/Main/Merge">005A22</idno>
<idno type="wicri:source">INIST</idno>
<idno type="RBID">Pascal:04-0524940</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000800</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000190</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000856</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000856</idno>
<idno type="wicri:doubleKey">0022-1899:2004:Liu I:disease:specific:b</idno>
<idno type="wicri:Area/Main/Merge">006007</idno>
<idno type="wicri:Area/Main/Curation">005576</idno>
<idno type="wicri:Area/Main/Exploration">005576</idno>
</publicationStmt>
<sourceDesc>
<biblStruct>
<analytic>
<title level="a" type="main">Disease-specific B cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens</title>
<author>
<name sortKey="Liu, I Ju" sort="Liu, I Ju" uniqKey="Liu I" first="I-Ju" last="Liu">I-Ju Liu</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Oral Biology, College of Medicine, National Taiwan UniversityTaipei</wicri:regionArea>
<wicri:noRegion>National Taiwan UniversityTaipei</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Laboratory Medicine and Internal Medicine, College of Medicine, National Taiwan UniversityTaipei</wicri:regionArea>
<wicri:noRegion>National Taiwan UniversityTaipei</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Lin, Chin Tarng" sort="Lin, Chin Tarng" uniqKey="Lin C" first="Chin-Tarng" last="Lin">Chin-Tarng Lin</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Pathology, College of Medicine, National Taiwan UniversityTaipei</wicri:regionArea>
<wicri:noRegion>National Taiwan UniversityTaipei</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Chiu, Chien Yu" sort="Chiu, Chien Yu" uniqKey="Chiu C" first="Chien-Yu" last="Chiu">Chien-Yu Chiu</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Oral Biology, College of Medicine, National Taiwan UniversityTaipei</wicri:regionArea>
<wicri:noRegion>National Taiwan UniversityTaipei</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>School and Graduate Institute of Medical Technology, College of Medicine, National Taiwan UniversityTaipei</wicri:regionArea>
<wicri:noRegion>National Taiwan UniversityTaipei</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Liao, Mei Ying" sort="Liao, Mei Ying" uniqKey="Liao M" first="Mei-Ying" last="Liao">Mei-Ying Liao</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Oral Biology, College of Medicine, National Taiwan UniversityTaipei</wicri:regionArea>
<wicri:noRegion>National Taiwan UniversityTaipei</wicri:noRegion>
</affiliation>
</author>
<author>
<name sortKey="Wu, Han Chung" sort="Wu, Han Chung" uniqKey="Wu H" first="Han-Chung" last="Wu">Han-Chung Wu</name>
<affiliation wicri:level="1">
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Oral Biology, College of Medicine, National Taiwan UniversityTaipei</wicri:regionArea>
<wicri:noRegion>National Taiwan UniversityTaipei</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1">
<country wicri:rule="url">Taïwan</country>
<wicri:regionArea>Reprints or correspondence: Dr. Han-Chung Wu, Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, 1 Chang-Te St., Taipei 100</wicri:regionArea>
<wicri:noRegion>Taipei 100</wicri:noRegion>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series>
<title level="j" type="main">Journal of Infectious Diseases</title>
<title level="j" type="abbrev">J Infect Dis.</title>
<idno type="ISSN">0022-1899</idno>
<idno type="eISSN">1537-6613</idno>
<imprint>
<publisher>The University of Chicago Press</publisher>
<date type="published">2004</date>
<biblScope unit="vol">190</biblScope>
<biblScope unit="issue">4</biblScope>
<biblScope unit="page" from="797">797</biblScope>
<biblScope unit="page" to="809">809</biblScope>
</imprint>
<idno type="ISSN">0022-1899</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt>
<idno type="ISSN">0022-1899</idno>
</seriesStmt>
</fileDesc>
<profileDesc>
<textClass>
<keywords scheme="KwdEn" xml:lang="en">
<term>Amino Acid Motifs (immunology)</term>
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies, Viral (blood)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antibody</term>
<term>Antigen</term>
<term>Antigen-Antibody Reactions</term>
<term>Antigenic determinant</term>
<term>Antigens, Viral (immunology)</term>
<term>B-Lymphocyte</term>
<term>B-Lymphocytes (immunology)</term>
<term>Chlorocebus aethiops</term>
<term>Detection</term>
<term>Epitopes (immunology)</term>
<term>Epitopes (isolation & purification)</term>
<term>Human</term>
<term>Humans</term>
<term>Microbiology</term>
<term>Molecular Sequence Data</term>
<term>Peptide Library</term>
<term>Peptides</term>
<term>Peptides (immunology)</term>
<term>Peptides (isolation & purification)</term>
<term>SARS Virus (immunology)</term>
<term>Sequence Alignment</term>
<term>Serology</term>
<term>Serum</term>
<term>Severe Acute Respiratory Syndrome (blood)</term>
<term>Severe Acute Respiratory Syndrome (etiology)</term>
<term>Severe Acute Respiratory Syndrome (immunology)</term>
<term>Severe acute respiratory syndrome</term>
<term>Species Specificity</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr">
<term>Alignement de séquences</term>
<term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (sang)</term>
<term>Antigènes viraux (immunologie)</term>
<term>Banque de peptides</term>
<term>Cellules Vero</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Lymphocytes B (immunologie)</term>
<term>Motifs d'acides aminés (immunologie)</term>
<term>Peptides (immunologie)</term>
<term>Peptides (isolement et purification)</term>
<term>Réaction antigène-anticorps</term>
<term>Spécificité d'espèce</term>
<term>Syndrome respiratoire aigu sévère (immunologie)</term>
<term>Syndrome respiratoire aigu sévère (sang)</term>
<term>Syndrome respiratoire aigu sévère (étiologie)</term>
<term>Séquence d'acides aminés</term>
<term>Virus du SRAS (immunologie)</term>
<term>Épitopes (immunologie)</term>
<term>Épitopes (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en">
<term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="blood" xml:lang="en">
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="etiology" xml:lang="en">
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr">
<term>Anticorps antiviraux</term>
<term>Antigènes viraux</term>
<term>Lymphocytes B</term>
<term>Motifs d'acides aminés</term>
<term>Peptides</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Virus du SRAS</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en">
<term>Amino Acid Motifs</term>
<term>Antibodies, Viral</term>
<term>Antigens, Viral</term>
<term>B-Lymphocytes</term>
<term>Epitopes</term>
<term>Peptides</term>
<term>SARS Virus</term>
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en">
<term>Epitopes</term>
<term>Peptides</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr">
<term>Peptides</term>
<term>Épitopes</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr">
<term>Anticorps antiviraux</term>
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" qualifier="étiologie" xml:lang="fr">
<term>Syndrome respiratoire aigu sévère</term>
</keywords>
<keywords scheme="MESH" xml:lang="en">
<term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antigen-Antibody Reactions</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Peptide Library</term>
<term>Sequence Alignment</term>
<term>Species Specificity</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr">
<term>Alignement de séquences</term>
<term>Animaux</term>
<term>Banque de peptides</term>
<term>Cellules Vero</term>
<term>Données de séquences moléculaires</term>
<term>Humains</term>
<term>Réaction antigène-anticorps</term>
<term>Spécificité d'espèce</term>
<term>Séquence d'acides aminés</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr">
<term>Anticorps</term>
<term>Antigène</term>
<term>Détection</term>
<term>Déterminant antigénique</term>
<term>Homme</term>
<term>Lymphocyte B</term>
<term>Microbiologie</term>
<term>Peptide</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Sérologie</term>
<term>Sérum</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr">
<term>Homme</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front>
<div type="abstract">Severe acute respiratory syndrome (SARS) has emerged as a highly contagious, sometimes fatal disease. To find disease-specific B cell epitopes, phage-displayed random peptide libraries were panned on serum immunoglobulin (Ig) G antibodies from patients with SARS. Forty-nine immunopositive phage clones that bound specifically to serum from patients with SARS were selected. These phageborne peptides had 4 consensus motifs, of which 2 corresponded to amino acid sequences reported for SARS-associated coronavirus (SARSCoV). Synthetic peptide binding and competitive-inhibition assays further confirmed that patients with SARS generated antibodies against SARS-CoV. Immunopositive phage clones and epitope-based peptide antigens demonstrated clinical diagnostic potential by reacting with serum from patients with SARS. Antibody-response kinetics were evaluated in 4 patients with SARS, and production of IgM, IgG, and IgA were documented as part of the immune response. In conclusion, B cell epitopes of SARS corresponded to novel coronavirus. Our epitope-based serologic test may be useful in laboratory detection of the virus and in further study of the pathogenesis of SARS.</div>
</front>
</TEI>
<affiliations>
<list>
<country>
<li>Taïwan</li>
</country>
</list>
<tree>
<country name="Taïwan">
<noRegion>
<name sortKey="Liu, I Ju" sort="Liu, I Ju" uniqKey="Liu I" first="I-Ju" last="Liu">I-Ju Liu</name>
</noRegion>
<name sortKey="Chiu, Chien Yu" sort="Chiu, Chien Yu" uniqKey="Chiu C" first="Chien-Yu" last="Chiu">Chien-Yu Chiu</name>
<name sortKey="Hsueh, Po Ren" sort="Hsueh, Po Ren" uniqKey="Hsueh P" first="Po-Ren" last="Hsueh">Po-Ren Hsueh</name>
<name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name>
<name sortKey="Liao, Mei Ying" sort="Liao, Mei Ying" uniqKey="Liao M" first="Mei-Ying" last="Liao">Mei-Ying Liao</name>
<name sortKey="Lin, Chin Tarng" sort="Lin, Chin Tarng" uniqKey="Lin C" first="Chin-Tarng" last="Lin">Chin-Tarng Lin</name>
<name sortKey="Wu, Han Chung" sort="Wu, Han Chung" uniqKey="Wu H" first="Han-Chung" last="Wu">Han-Chung Wu</name>
<name sortKey="Wu, Han Chung" sort="Wu, Han Chung" uniqKey="Wu H" first="Han-Chung" last="Wu">Han-Chung Wu</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005576 | SxmlIndent | more

Ou

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005576 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     ISTEX:C2A0D758774998C51462273113E7DFDB9C630BC7
   |texte=   Disease-specific B cell epitopes for serum antibodies from patients with severe acute respiratory syndrome (SARS) and serologic detection of SARS antibodies by epitope-based peptide antigens
}}

Wicri

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021